Anti-SMARCA2/BRM Antibody Picoband® (monoclonal, 4E8)

SKU: M01888-1

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human

Applications: IF,ICC,WB

Application Details: Western blot, 0.1-0.5μg/ml, Human
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human

Gene Name: SMARCA2

Specificity: No cross reactivity with other proteins.

Background: Probable global transcription activator SNF2L2 is a protein that in humans is encoded by the SMARCA2 gene. It is mapped to 9p24.3. The protein encoded by this gene is a member of the SWI/SNF family of proteins and is highly similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. Alternatively spliced transcript variants encoding different isoforms have been found for this gene, which contains a trinucleotide repeat (CAG) length polymorphism.

Immunogen: E.coli-derived human SMARCA2/BRM recombinant protein (Position: Q181-N624).

Clonality: Monoclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. de la Serna, I. L., Carlson, K. A., Imbalzano, A. N. Mammalian SWI/SNF complexes promote MyoD-mediated muscle differentiation. Nature Genet. 27: 187-190, 2001. 2. Hakimi, M.-A., Bochar, D. A., Schmiesing, J. A., Dong, Y., Barak, O. G., Speicher, D. W., Yokomori, K., Shiekhattar, R. A chromatin remodelling complex that loads cohesin onto human chromosomes. Nature 418: 994-998, 2002.

Uniprot ID: P51531

Host: Mouse

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Mouse IgG2b

Phospho_site:

Clone Number: Clone: 4E8

Observed Molecular Weight: 210 kDa

Calculated Molecular Weight: 25035 MW

Gene ID: 6595

Protein Name: SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 2

Gene Full Name: SWI/SNF related BAF chromatin remodeling complex subunit ATPase 2

Synonyms: ATP dependent helicase SMARCA2; BAF190; BAF190B; BRG1 associated factor 190B; BRM; hBRM; hSNF2a; Protein brahma homolog; SMARCA2; SMARCA2-Specific; SNF2; SNF2 alpha; SNF2A; SNF2L2; SNF2LA; Sth1p; SWI2