Anti-Smad3 Antibody Picoband®

SKU: PB9396

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: ICC/IF,IHC,WB

Application Details: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human

Gene Name: SMAD3

Specificity: No cross reactivity with other proteins.

Background: Mothers against decapentaplegic homolog 3 also known as SMAD family member 3 or SMAD3 is a protein that in humans is encoded by the SMAD3 gene. SMAD3 is a member of the SMAD family of proteins. It is located in 15q22.33. And it mediates the signals from the transforming growth factor beta (TGF-β) super family ligands that regulate cell proliferation, differentiation and death. Based on its essential role in TGF beta signaling pathway, SMAD3 has been related with tumor growth in cancer development.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human Smad3, identical to the related mouse and rat sequences.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Entrez Gene: SMAD3 SMAD family member 3. 2. Moustakas A, Souchelnytskyi S, Heldin CH (2001). Smad regulation in TGF-beta signal transduction. J. Cell. Sci. 114 (Pt 24): 4359–69. 3. Zhang Y, Feng X, We R, Derynck R (September 1996). Receptor-associated Mad homologues synergize as effectors of the TGF-beta response. Nature 383 (6596): 168–72.

Uniprot ID: P84022

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 50-55 kDa

Calculated Molecular Weight: 48 kDa

Gene ID: 4088

Protein Name: Mothers against decapentaplegic homolog 3

Gene Full Name: SMAD family member 3

Synonyms: hMAD 3; hSMAD3; HSPC193; HsT17436; JV15 2; MAD homolog 3; Mad3; MADH3; Mothers against DPP homolog 3; SMAD 3; SMAD family member 3; SMAD3