Anti-SKP2 Antibody Picoband®

SKU: PA1102

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human

Applications: Flow Cytometry,ICC/IF,WB

Application Details: Western blot, 0.1-0.5μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human

Gene Name: SKP2

Specificity: No cross reactivity with other proteins.

Background: The F box protein Skp2 (S-phase kinase-associated protein 2) is oncogenic, and its frequent amplification and overexpression correlate with the grade of malignancy of certain tumors. Skp2 controls p300-p53 signaling pathways in cancer cells, making it a potential molecular target for cancer therapy. This gene positively regulates the G (1)-S transition by controlling the stability of several G (1) regulators, such as the cell cycle inhibitor p27. This study provides evidence of a role for an F-box protein in oncogenesis and establishes SKP2 as a protooncogene causally involved in the pathogenesis of lymphomas.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human SKP2, different from the related rat and mouse sequences by three amino acids.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Kitagawa, M.; Lee, S. H.; McCormick, F. : Skp2 suppresses p53-dependent apoptosis by inhibiting p300. Molec. Cell 29: 217-231, 2008. 2. Latres, E.; Chiarle, R.; Schulman, B. A.; Pavletich, N. P.; Pellicer, A.; Inghirami, G.; Pagano, M. : Role of the F-box protein Skp2 in lymphomagenesis. Proc. Nat. Acad. Sci. 98: 2515-2520, 2001.

Uniprot ID: Q13309

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 48 kDa

Calculated Molecular Weight: 48 kDa

Gene ID: 6502

Protein Name: S-phase kinase-associated protein 2

Gene Full Name: S-phase kinase associated protein 2

Synonyms: F box protein Skp2; F box/LRR repeat protein 1; FBL1; FBXL1; FLB1; p45skp2; SKP2