Anti-Sex Hormone Binding Globulin/SHBG Antibody Picoband®

SKU: PB9326

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human

Applications: WB

Application Details: Western blot, 0.1-0.5μg/ml, Human

Gene Name: SHBG

Specificity: No cross reactivity with other proteins.

Background: Sex hormone-binding globulin (SHBG) is a glycoprotein that binds to the sex hormones,androgen and estrogen. SHBG is produced mostly by the liver and is released into the bloodstream. Other sites that produce SHBG include the brain, uterus, testes, and placenta. SHBG has both enhancing and inhibiting hormonal influences. It decreases with high levels of insulin, growth hormone, insulin-like growth factor 1 (IGF-1), androgens, prolactin and transcortin. High estrogen, andthyroxine cause it to increase. It has been found that the monosaccharides glucose and fructose reduced human SHBG production by hepatocytes, irrespective of the presence of insulin, by downregulation of hepatocyte nuclear factor-4A and replacement of HNF4A by COUP-TFI at a cis-element within the human SHBG promoter, coincident with repression of its transcriptional activity.

Immunogen: E.coli-derived human SHBG recombinant protein (Position: L30-A210). Human SHBG shares 72% amino acid (aa) sequence identity with both mouse and rat SHBG.

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Hammond GL (2011). Diverse Roles for Sex Hormone-Binding Globulin in Reproduction. Biology of Reproduction 85(3): 431–441. 2. Selva, D. M., Hogeveen, K. N., Innis, S. M., Hammond, G. L. Monosaccharide-induced lipogenesis regulates the human hepatic sex hormone-binding globulin gene. J. Clin. Invest. 117: 3979-3987, 2007. 3. Simó R, Barbosa-Desongles A, Hernandez C, Selva DM (November 2012). IL1β down-regulation of sex hormone-binding globulin production by decreasing HNF-4α via MEK-1/2 and JNK MAPK pathways. Mol. Endocrinol. 26 (11): 1917–27.

Uniprot ID: P04278

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 70 kDa

Calculated Molecular Weight: 43779 MW

Gene ID: 6462

Protein Name: Sex hormone-binding globulin

Gene Full Name: sex hormone binding globulin

Synonyms: ABP; Androgen binding protein; SBP; sex hormone binding globulin; Sex steroid binding protein; SHBG; TEBG