Anti-RRN3 Antibody Picoband®

SKU: A05439-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: RRN3

Specificity:

Background: RNA polymerase I-specific transcription initiation factor RRN3 is an enzyme that in humans is encoded by the RRN3 gene. This gene encodes a member of the cytochrome P450 superfamily of enzymes, which participate in drug metabolism and the synthesis of cholesterol, steroids and other lipids. This enzyme hydroxylates testosterone and dehydroepiandrosterone 3-sulphate, which is involved in the formation of estriol during pregnancy. This gene is part of a cluster of related genes on chromosome 7q21.1.

Immunogen: E.coli-derived human RRN3 recombinant protein (Position: D184-L651). Human RRN3 shares 87.4% amino acid (aa) sequence identity with mouse RRN3.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Mayer, C., Bierhoff, H., Grummt, I. The nucleolus as a stress sensor: JNK2 inactivates the transcription factor TIF-IA and down-regulates rRNA synthesis. Genes Dev. 19: 933-941, 2005. 2. Moorefield, B., Greene, E. A., Reeder, R. H. RNA polymerase I transcription factor Rrn3 is functionally conserved between yeast and human. Proc. Nat. Acad. Sci. 97: 4724-4729, 2000. 3. Yuan, X., Zhou, Y., Casanova, E., Chai, M., Kiss, E., Grone, H.-J., Schutz, G., Grummt, I. Genetic inactivation of the transcription factor TIF-IA leads to nucleolar disruption, cell cycle arrest, and p53-mediated apoptosis. Molec. Cell 19: 77-87, 2005.

Uniprot ID: Q9NYV6

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity:

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 74 kDa

Calculated Molecular Weight: 74 kDa

Gene ID: 54700

Protein Name: RNA polymerase I-specific transcription initiation factor RRN3

Gene Full Name: RNA polymerase I transcription factor RRN3

Synonyms: RRN3; TIFIA; RNA polymerase I-specific transcription initiation factor RRN3; Transcription initiation factor IA; TIF-IA