Anti-RPS3A Antibody Picoband®

SKU: A05268-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,ICC/IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: RPS3A

Specificity:

Background: 40S ribosomal protein S3a is a protein that in humans is encoded by the RPS3A gene. Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S3AE family of ribosomal proteins. It is located in the cytoplasm. Disruption of the gene encoding rat ribosomal protein S3a, also named v-fos transformation effector protein, in v-fos-transformed rat cells results in reversion of the transformed phenotype. This gene is co-transcribed with the U73A and U73B small nucleolar RNA genes, which are located in its fourth and third introns, respectively. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Alternatively spliced transcript variants have been found for this gene.

Immunogen: E.coli-derived human RPS3A recombinant protein (Position: K18-R146). Human RPS3A shares 100% amino acid (aa) sequence identity with both mouse and rat RPS3A.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Kenmochi, N., Kawaguchi, T., Rozen, S., Davis, E., Goodman, N., Hudson, T. J., Tanaka, T., Page, D. C. A map of 75 human ribosomal protein genes. Genome Res. 8: 509-523, 1998. 2. Kho, C. J., Wang, Y., Zarbl, H. Effect of decreased fte-1 gene expression on protein synthesis, cell growth, and transformation. Cell Growth Differ. 7: 1157-1166, 1996. 3. Metspalu, A., Rebane, A., Hoth, S., Pooga, M., Stahl, J., Kruppa, J. Human ribosomal protein S3a: cloning of the cDNA and primary structure of the protein. Gene 119: 313-316, 1992.

Uniprot ID: P61247

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity:

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 35 kDa

Calculated Molecular Weight: 30 kDa

Gene ID: 6189

Protein Name: Small ribosomal subunit protein eS1

Gene Full Name: ribosomal protein S3A

Synonyms: RPS3A; FTE1; MFTL; 40S ribosomal protein S3a; Small ribosomal subunit protein eS1; v-fos transformation effector protein; Fte-1