Anti-RNF220 Antibody Picoband®

SKU: A11818-1

Size: 100 μg

Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,ICC/IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml

Gene Name: RNF220

Specificity:

Background: Enables beta-catenin binding activity. Involved in positive regulation of canonical Wnt signaling pathway. Acts upstream of or within positive regulation of DNA-binding transcription factor activity and protein monoubiquitination. Located in nuclear lamina. Part of protein-containing complex. Implicated in hypomyelinating leukodystrophy 23.

Immunogen: E.coli-derived human RNF220 recombinant protein (Position: M1-L566). Human RNF220 shares 97.9% amino acid (aa) sequence identity with mouse RNF220.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Hartz, P. A. Personal Communication. Baltimore, Md. 12/12/2014. 2. Kong, Q., Zeng, W., Wu, J., Hu, W., Li, C., Mao, B. RNF220, an E3 ubiquitin ligase that targets Sin3B for ubiquitination. Biochem. Biophys. Res. Commun. 393: 708-713, 2010. 3. Leuzzi, V., Rinna, A., Gallucci, M., Di Capua, M., Dionisi-Vici, C., Longo, D., Bertini, E. Ataxia, deafness, leukodystrophy: inherited disorder of the white matter in three related patients. Neurology 54: 2325-2328, 2000.

Uniprot ID: Q5VTB9

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 63 kDa

Calculated Molecular Weight: 63 kDa

Gene ID: 55182

Protein Name: E3 ubiquitin-protein ligase RNF220

Gene Full Name: ring finger protein 220

Synonyms: RNF220; C1orf164; E3 ubiquitin-protein ligase RNF220; EC 2.3.2.27; RING finger protein 220; RING-type E3 ubiquitin transferase RNF220