Anti-RNA Helicase A/DHX9 Antibody Picoband®

SKU: A02550-1

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: ELISA,Flow Cytometry,IP,IF,IHC,ICC,WB

Application Details: Western blot, 0.1-0.25μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Immunofluorescence, 2μg/ml, Human
Immunoprecipitation, 0.5-2 μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human
ELISA, 0.1-0.5μg/ml, -

Gene Name: DHX9

Specificity: No cross reactivity with other proteins.

Background: ATP-dependent RNA helicase A (RHA; also known as DHX9, LKP, and NDHI) is an enzyme that in humans is encoded by the DHX9 gene. It is mapped to 1q25.3. This gene encodes a member of the DEAH-containing family of RNA helicases. The encoded protein is an enzyme that catalyzes the ATP-dependent unwinding of double-stranded RNA and DNA-RNA complexes. This protein localizes to both the nucleus and the cytoplasm and functions as a transcriptional regulator. This protein may also be involved in the expression and nuclear export of retroviral RNAs. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 11 and 13.

Immunogen: E.coli-derived human RNA Helicase A/DHX9 recombinant protein (Position: E396-E759).

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Abdelhaleem, M. M., Hameed, S., Klassen, D., Greenberg, A. H. Leukophysin: an RNA helicase A-related molecule identified in cytotoxic T cell granules and vesicles. J. Immun. 156: 2026-2035, 1996. 2. Aktas, T., Avsar Ilik, I., Maticzka, D., Bhardwaj, V., Pessoa Rodrigues, C., Mittler, G., Manke, T., Backofen, R., Akhtar, A. DHX9 suppresses RNA processing defects originating from the Alu invasion of the human genome. Nature 544: 115-119, 2017. 3. Lee, C. G., Hurwitz, J. A new RNA helicase isolated from HeLa cells that catalytically translocates in the 3-prime to 5-prime ion. J. Biol. Chem. 267: 4398-4407, 1992.

Uniprot ID: Q08211

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 141 kDa

Calculated Molecular Weight:

Gene ID: 1660

Protein Name: ATP-dependent RNA helicase A

Gene Full Name: DExH-box helicase 9

Synonyms: ATP dependent RNA helicase A; DDX9; DEAH box protein 9; DHX9; LKP; NDH II; NDH2; NDHII; Nuclear DNA helicase II; RHA