Anti-RFXANK Antibody Picoband®

SKU: A07934

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: RFXANK

Specificity:

Background: DNA-binding protein RFXANK is a protein that in humans is encoded by the RFXANK gene. Major histocompatibility (MHC) class II molecules are transmembrane proteins that have a central role in development and control of the immune system. The protein encoded by this gene, along with regulatory factor X-associated protein and regulatory factor-5, forms a complex that binds to the X box motif of certain MHC class II gene promoters and activates their transcription. Once bound to the promoter, this complex associates with the non-DNA-binding factor MHC class II transactivator, which controls the cell type specificity and inducibility of MHC class II gene expression. This protein contains ankyrin repeats involved in protein-protein interactions. Mutations in this gene have been linked to bare lymphocyte syndrome type II, complementation group B. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Immunogen: E.coli-derived human RFXANK recombinant protein (Position: E29-E260). Human RFXANK shares 85.1% amino acid (aa) sequence identity with mouse RFXANK.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Lennon-Dumenil, A.-M., Barbouche, M.-R., Vedrenne, J., Prod'Homme, T., Bejaoui, M., Ghariani, S., Charron, D., Fellous, M., Dellagi, K., Alcaide-Loridan, C. Uncoordinated HLA-D gene expression in a RFXANK-defective patient with MHC class II deficiency. J. Immun. 166: 5681-5687, 2001. 2. Lin, J. H., Makris, A., McMahon, C., Bear, S. E., Patriotis, C., Prasad, V. R., Brent, R., Golemis, E. A., Tsichlis, P. N. The ankyrin repeat-containing adaptor protein Tvl-1 is a novel substrate and regulator of Raf-1. J. Biol. Chem. 274: 14706-14715, 1999. 3. Lisowska-Grospierre, B., Fondaneche, M. C., Rols, M. P., Griscelli, C., Fischer, A. Two complementation groups account for most cases of inherited MHC class II deficiency. Hum. Molec. Genet. 3: 953-958, 1994.

Uniprot ID: O14593

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross reactivity with other proteins.

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 34 kDa

Calculated Molecular Weight: 52588 MW

Gene ID: 8625

Protein Name: DNA-binding protein RFXANK

Gene Full Name: regulatory factor X associated ankyrin containing protein

Synonyms: DNA-binding protein RFXANK; Ankyrin repeat family A protein 1; Regulatory factor X subunit B; RFX-B; RFXANK; ANKRA1; RFXB; BLS; F14150_1