Anti-RBBP8 Antibody Picoband®

SKU: A02076-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse

Applications: WB,IHC,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: RBBP8

Specificity:

Background: Retinoblastoma-binding protein 8 is a protein that in humans is encoded by the RBBP8 gene. The protein encoded by this gene is a ubiquitously expressed nuclear protein. It is found among several proteins that bind ly to retinoblastoma protein, which regulates cell proliferation. This protein complexes with transcriptional co-repressor CTBP. It is also associated with BRCA1 and is thought to modulate the functions of BRCA1 in transcriptional regulation, DNA repair, and/or cell cycle checkpoint control. It is suggested that this gene may itself be a tumor suppressor acting in the same pathway as BRCA1. Three transcript variants encoding two different isoforms have been found for this gene. More transcript variants exist, but their full-length natures have not been determined.

Immunogen: E.coli-derived human RBBP8 recombinant protein (Position: R307-K702). Human RBBP8 shares 68.9% and 66.7% amino acid (aa) sequence identity with mouse and rat RBBP8, respectively.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Borglum, A. D., Balslev, T., Haagerup, A., Birkebaek, N., Binderup, H., Kruse, T. A., Hertz, J. M. A new locus for Seckel syndrome on chromosome 18p11.31-q11.2. Europ. J. Hum. Genet. 9: 753-757, 2001. 2. Fusco, C., Reymond, A., Zervos, A. S. Molecular cloning and characterization of a novel retinoblastoma-binding protein. Genomics 51: 351-358, 1998. 3. Hassan, M. J., Chishti, M. S., Jamal, S. M., Tariq, M., Ahmad, W. A syndromic form of autosomal recessive congenital microcephaly (Jawad syndrome) maps to chromosome 18p11.22-q11.2. Hum. Genet. 123: 77-82, 2008.

Uniprot ID: Q99708

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross reactivity with other proteins.

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 130 kDa

Calculated Molecular Weight: 52588 MW

Gene ID: 5932

Protein Name: DNA endonuclease RBBP8

Gene Full Name: RB binding protein 8, endonuclease

Synonyms: RBBP8; CTIP; DNA endonuclease RBBP8; CtBP-interacting protein; CtIP; Retinoblastoma-binding protein 8; RBBP-8; Retinoblastoma-interacting protein and myosin-like; RIM; Sporulation in the absence of SPO11 protein 2 homolog; SAE2