Anti-RAP1GAP Antibody Picoband®

SKU: A03561-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: RAP1GAP

Specificity:

Background: Rap1 GTPase-activating protein 1 is an enzyme that in humans is encoded by the RAP1GAP gene. This gene encodes a type of GTPase-activating-protein (GAP) that down-regulates the activity of the ras-related RAP1 protein. RAP1 acts as a molecular switch by cycling between an inactive GDP-bound form and an active GTP-bound form. The product of this gene, RAP1GAP, promotes the hydrolysis of bound GTP and hence returns RAP1 to the inactive state whereas other proteins, guanine nucleotide exchange factors (GEFs), act as RAP1 activators by facilitating the conversion of RAP1 from the GDP- to the GTP-bound form. In general, ras subfamily proteins, such as RAP1, play key roles in receptor-linked signaling pathways that control cell growth and differentiation. RAP1 plays a role in diverse processes such as cell proliferation, adhesion, differentiation, and embryogenesis. Alternative splicing results in multiple transcript variants encoding distinct proteins.

Immunogen: E.coli-derived human RAP1GAP recombinant protein (Position: D11-L661).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Daumke, O., Weyand, M., Chakrabarti, P. P., Vetter, I. R., Wittinghofer, A. The GTPase-activating protein Rap1GAP uses a catalytic asparagine. Nature 429: 197-201, 2004. 2. Rubinfeld, B., Munemitsu, S., Clark, R., Conroy, L., Watt, K., Crosier, W. J., McCormick, F., Polakis, P. Molecular cloning of a GTPase activating protein specific for the Krev-1 protein p21-rap1. Cell 65: 1033-1042, 1991. 3. Weiss, J., Rubinfeld, B., Polakis, P. G., McCormick, F., Cavenee, W. K., Arden, K. C. The RAP1GA1 locus for human Rap1-GTPase activating protein 1 maps to chromosome 1p36.1-p35. Cytogenet. Cell Genet. 66: 18-21, 1994.

Uniprot ID: P47736

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 95 kDa

Calculated Molecular Weight: 75187 MW

Gene ID: 5909

Protein Name: Rap1 GTPase-activating protein 1

Gene Full Name: RAP1 GTPase activating protein

Synonyms: RAP1GA1; Rap1GAP; Rap1GAP1; RAP1GAPII; RAPGAP