Anti-PUM1 Antibody Picoband®

SKU: A02111-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: PUM1

Specificity:

Background: Pumilio homolog 1 is a protein that in humans is encoded by the PUM1 gene. This gene encodes a member of the PUF family, evolutionarily conserved RNA-binding proteins related to the Pumilio proteins of Drosophila and the fem-3 mRNA binding factor proteins of C. elegans. The encoded protein contains a sequence-specific RNA binding domain comprised of eight repeats and N- and C-terminal flanking regions, and serves as a translational regulator of specific mRNAs by binding to their 3' untranslated regions. The evolutionarily conserved function of the encoded protein in invertebrates and lower vertebrates suggests that the human protein may be involved in translational regulation of embryogenesis, and cell development and differentiation. Alternatively spliced transcript variants encoding different isoforms have been described.

Immunogen: E.coli-derived human PUM1 recombinant protein (Position: M1-D361).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Bonnemason-Carrere, P., Morice-Picard, F., Pennamen, P., Arveiler, B., Fergelot, P., Goizet, C., Hellegouarch, M., Lacombe, D., Plaisant, C., Raclet, V., Rooryck, C., Lasseaux, E., Trimouille, A. PADDAS syndrome associated with hair dysplasia caused by a de novo missense variant of PUM1. Am. J. Med. Genet. 179A: 1030-1033, 2019. 2. Gennarino, V. A., Palmer, E. E., McDonell, L. M., Wang, L., Adamski, C. J., Koire, A., See, L., Chen, C.-A., Schaaf, C. P., Rosenfeld, J. A., Panzer, J. A., Moog, U. A mild PUM1 mutation is associated with adult-onset ataxia, whereas haploinsufficiency causes developmental delay and seizures. Cell 172: 924-936, 2018. 3. Gennarino, V. A., Singh, R. K., White, J. J., De Maio, A., Han, K., Kim, J.-Y., Jafar-Nejad, P., di Ronza, A., Kang, H., Sayegh, L. S., Cooper, T. A., Orr, H. T., Sillitoe, R. V., Zoghbi, H. Y. Pumilio1 haploinsufficiency leads to SCA1-like neurodegeneration by increasing wild-type ataxin1 levels. Cell 160: 1087-1098, 2015.

Uniprot ID: Q14671

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity:

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 140 kDa

Calculated Molecular Weight: 126 kDa

Gene ID: 9698

Protein Name: Pumilio homolog 1

Gene Full Name: pumilio RNA binding family member 1

Synonyms: HSPUM; KIAA0099; PUM1; PUMH; PUMH1; Pumilio 1; Pumilio homolog 1; pumilio homolog 1 (Drosophila); PUML1