Anti-PTPN12 Antibody Picoband®

SKU: A02762-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: PTPN12

Specificity:

Background: Tyrosine-protein phosphatase non-receptor type 12 is an enzyme that in humans is encoded by the PTPN12 gene. The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains a C-terminal PEST motif, which serves as a protein-protein interaction domain, and may regulate protein intracellular half-life. This PTP was found to bind and dephosphorylate the product of the oncogene c-ABL and thus may play a role in oncogenesis. This PTP was also shown to interact with, and dephosphorylate, various products related to cytoskeletal structure and cell adhesion, such as p130 (Cas), CAKbeta/PTK2B, PSTPIP1, and paxillin. This suggests it has a regulatory role in controlling cell shape and mobility. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Immunogen: E.coli-derived human PTPN12 recombinant protein (Position: A276-D311).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Charest, A., Wagner, J., Muise, E. S., Heng, H. H. Q., Tremblay, M. L. Structure of the murine MPTP-PEST gene: genomic organization and chromosomal mapping. Genomics 28: 501-507, 1995. 2. Cong, F., Spencer, S., Cote, J.-F., Wu, Y., Tremblay, M. L., Lasky, L. A., Goff, S. P. Cytoskeletal protein PSTPIP1 s the PEST-type protein tyrosine phosphatase to the c-Abl kinase to mediate Abl dephosphorylation. Molec. Cell 6: 1413-1423, 2000. 3. Spencer, S., Dowbenko, D., Cheng, J., Li, W., Brush, J., Utzig, S., Simanis, V., Lasky, L. A. PSTPIP: a tyrosine phosphorylated cleavage furrow-associated protein that is a substrate for PEST tyrosine phosphatase. J. Cell Biol. 138: 845-860, 1997.

Uniprot ID: Q05209

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 110 kDa

Calculated Molecular Weight: 12252 MW

Gene ID: 5782

Protein Name: Tyrosine-protein phosphatase non-receptor type 12

Gene Full Name: protein tyrosine phosphatase non-receptor type 12

Synonyms: PTPN12; Tyrosine-protein phosphatase non-receptor type 12; EC 3.1.3.48; PTP-PEST; Protein-tyrosine phosphatase G1; PTPG1