Anti-PSIP1 Antibody Picoband®

SKU: A01960-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: ELISA,IF,IHC,ICC,WB,Flow Cytometry

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: PSIP1

Specificity:

Background: PC4 and SFRS1 interacting protein 1, also known as lens epithelium-derived growth factor (LEDGF/p75), dense fine speckles 70kD protein (DFS 70) or transcriptional coactivator p75/p52, is a protein that in humans is encoded by the PSIP1 gene. PSIP1 is a chromatin associated protein that is shown to act as a transcriptional coactivator as well as an RNA-binding protein. PSIP1 encodes two protein isoforms with molecular masses of 52 and 75 kDa. p52 and p75 were identified as interacting with transcription factor PC4 and shown to act as transcriptional coactivators.

Immunogen: E.coli-derived human PSIP1 recombinant protein (Position: D280-N530).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Cherepanov, P., Ambrosio, A. L. B., Rahman, S., Ellenberger, T., Engelman, A. Structural basis for the recognition between HIV-1 integrase and transcriptional coactivator p75. Proc. Nat. Acad. Sci. 102: 17308-17313, 2005. 2. Ciuffi, A., Llano, M., Poeschla, E., Hoffmann, C., Leipzig, J., Shinn, P., Ecker, J. R., Bushman, F. A role for LEDGF/p75 in targeting HIV DNA integration. Nature Med. 11: 1287-1289, 2005. 3. Ge, H., Si, Y., Roeder, R. G. Isolation of cDNAs encoding novel transcription coactivators p52 and p75 reveals an alternate regulatory mechanism of transcriptional activation. EMBO J. 17: 6723-6729, 1998.

Uniprot ID: O75475

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross reactivity with other proteins.

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 85 kDa

Calculated Molecular Weight: 52588 MW

Gene ID: 11168

Protein Name: PC4 and SFRS1-interacting protein

Gene Full Name: PC4 and SRSF1 interacting protein 1

Synonyms: CLL associated antigen KW 7; DFS 70; DFS70; LEDGF; p52; p75; PAIP; PSIP1; PSIP2