Anti-Proteasome 20S C2/PSMA1 Antibody Picoband®

SKU: PB10087

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: IHC,WB

Application Details: Western blot, 0.1-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human

Gene Name: PSMA1

Specificity: No cross reactivity with other proteins.

Background: Proteasome subunit alpha type-1 is a protein that in humans is encoded by the PSMA1 gene. The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human PSMA1, different from the related mouse sequence by one amino acid, and from the related rat sequence by two amino acids.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Groll M, Ditzel L, Löwe J, Stock D, Bochtler M, Bartunik HD, Huber R (Apr 1997). Structure of 20S proteasome from yeast at 2.4 A resolution. Nature 386 (6624): 463–71. 2. Tomko RJ, Hochstrasser M (2013). Molecular architecture and assembly of the eukaryotic proteasome.Annual Review of Biochemistry 82: 415–45.

Uniprot ID: P25786

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 29 kDa

Calculated Molecular Weight: 29556 MW

Gene ID: 5682

Protein Name: Proteasome subunit alpha type-1

Gene Full Name: proteasome 20S subunit alpha 1

Synonyms: 20S proteasome; 30 kDa prosomal protein; HC2; Macropain subunit C2; NU; PROS 30; PROS30; Proteasome component C2; Proteasome nu chain; PSC2; PSMA1