Anti-Proteasome 20S beta 7/PSMB7 Antibody Picoband® (monoclonal, 3H6C8)

SKU: M08095-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Monkey,Mouse,Rat

Applications: WB,Flow Cytometry

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Monkey, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Gene Name: PSMB7

Specificity:

Background: Proteasome subunit beta type-7 as known as 20S proteasome subunit beta-2 is a protein that in humans is encoded by the PSMB7 gene. The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. The encoded protein is a member of the proteasome B-type family, also known as the T1B family, and is a 20S core beta subunit in the proteasome. Expression of this catalytic subunit is downregulated by gamma interferon, and proteolytic processing is required to generate a mature subunit. A pseudogene of this gene is located on the long arm of chromosome 14.

Immunogen: E.coli-derived human Proteasome 20S beta 7/PSMB7 recombinant protein (Position: D17-S277).

Clonality: Monoclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Coux, O., Tanaka, K., Goldberg, A. L. Structure and functions of the 20S and 26S proteasomes. Ann. Rev. Biochem. 65: 801-847, 1996. 2. Hisamatsu, H., Shimbara, N., Saito, Y., Kristensen, P., Hendil, K. B., Fujiwara, T., Takahashi, E., Tanahashi, N., Tamura, T., Ichihara, A., Tanaka, K. Newly identified pair of proteasomal subunits regulated reciprocally by interferon gamma. J. Exp. Med. 183: 1807-1816, 1996.

Uniprot ID: Q99436

Host: Mouse

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: IgG2b

Phospho_site:

Clone Number: Clone: 3H6C8

Observed Molecular Weight: 25 kDa

Calculated Molecular Weight:

Gene ID: 5695

Protein Name: Proteasome subunit beta type-7

Gene Full Name: proteasome 20S subunit beta 7

Synonyms: Z; PSMB7; Macropain chain Z; Proteasome subunit Z; Proteasome subunit beta type-7