Anti-Prosurfactant Protein B/SFTPB Antibody Picoband®

SKU: A03441-1

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB

Application Details: Western blot, 0.1-0.5μg/ml

Gene Name: SFTPB

Specificity: No cross reactivity with other proteins.

Background: Pulmonary surfactant-associated protein B is a protein that in humans is encoded by the SFTPB gene. This gene encodes the pulmonary-associated surfactant protein B (SPB), an amphipathic surfactant protein essential for lung function and homeostasis after birth. Pulmonary surfactant is a surface-active lipoprotein complex composed of 90% lipids and 10% proteins which include plasma proteins and apolipoproteins SPA, SPB, SPC and SPD. The surfactant is secreted by the alveolar cells of the lung and maintains the stability of pulmonary tissue by reducing the surface tension of fluids that coat the lung. The SPB enhances the rate of spreading and increases the stability of surfactant monolayers in vitro. Multiple mutations in this gene have been identified, which cause pulmonary surfactant metabolism dysfunction type 1, also called pulmonary alveolar proteinosis due to surfactant protein B deficiency, and are associated with fatal respiratory distress in the neonatal period. Alternatively spliced transcript variants encoding the same protein have been identified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human SFTPB, which shares 80% amino acid (aa) sequence identity with both mouse and rat SFTPB.

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Tredano, M., Cooper, D. N., Stuhrmann, M., Christodoulou, J., Chuzhanova, N. A., Roudot-Thoraval, F., Boelle, P.-Y., Elion, J., Jeanpierre, M., Feingold, J., Couderc, R., Bahuau, M. Origin of the prevalent SFTPB Indel g.1549C-GAA (121ins2) mutation causing surfactant protein B (SP-B) deficiency. Am. J. Med. Genet. 140A: 62-69, 2006. 2. Tredano, M., van Elburg, R. M., Kaspers, A. G., Zimmermann, L. J., Houdayer, C., Aymard, P., Hull, W. M., Whitsett, J. A., Elion, J., Griese, M., Bahuau, M. Compound SFTPB 1549C-to-GAA (121ins2) and 457delC heterozygosity in severe congenital lung disease and surfactant protein B (SP-B) deficiency. Hum. Mutat. 14: 502-509, 1999.

Uniprot ID: P07988

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 46 kDa

Calculated Molecular Weight: 31203 MW

Gene ID: 6439

Protein Name: Pulmonary surfactant-associated protein B

Gene Full Name: surfactant protein B

Synonyms: 6 kDa protein; PSP B; SFTB3; SFTP3; SFTPB; SMDP1; SP B; surfactant protein B