Anti-Prolyl Endopeptidase/PREP Antibody Picoband®

SKU: A00298-2

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Rat

Applications: WB,IHC,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Rat
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human
ELISA, 0.1-0.5μg/ml, -

Gene Name: PREP

Specificity:

Background: Prolyl endopeptidase (PE) also known as prolyl oligopeptidase or post-proline cleaving enzyme is an enzyme that in humans is encoded by the PREP gene. The protein encoded by this gene is a cytosolic prolyl endopeptidase that cleaves peptide bonds on the C-terminal side of prolyl residues within peptides that are up to approximately 30 amino acids long. Prolyl endopeptidases have been reported to be involved in the maturation and degradation of peptide hormones and neuropeptides.

Immunogen: E.coli-derived human PREP recombinant protein (Position: M1-E227).

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Fulop, V., Bocskei, Z., Polgar, L. Prolyl oligopeptidase: an unusual beta-propeller domain regulates proteolysis. Cell 94: 161-170, 1998. 2. Goossens, F. J., Wauters, J. G., Vanhoof, G. C., Bossuyt, P. J., Schatteman, K. A., Loens, K., Scharpe, S. L. Subregional mapping of the human lymphocyte prolyl oligopeptidase gene (PREP) to human chromosome 6q22. Cytogenet. Cell Genet. 74: 99-101, 1996. 3. Valdivia, A., Irazusta, J., Fernandez, D., Mugica, J., Ochoa, C., Casis, L. Pyroglutamyl peptidase I and prolyl endopeptidase in human semen: increased activity in necrozoospermia. Regulatory Peptides 122: 79-84, 2004.

Uniprot ID: P48147

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 81 kDa

Calculated Molecular Weight: 50495 MW

Gene ID: 5550

Protein Name: Prolyl endopeptidase

Gene Full Name: prolyl endopeptidase

Synonyms: PE; PEP; Post proline cleaving enzyme; PREP; prolyl endopeptidase