Anti-PRAG1 Antibody Picoband®

SKU: A32113

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: PRAG1

Specificity:

Background: This gene encodes an enzyme that belongs to the tyrosine protein kinase family. A similar protein in rat binds to Rho family GTPase 2 (Rnd2) and regulates neurite outgrowth via activation of Ras homolog gene family, member A (RhoA).

Immunogen: E.coli-derived human PRAG1 recombinant protein (Position: H2-V1104). Human PRAG1 shares 74.1% and 73.4% amino acid (aa) sequence identity with mouse and rat PRAG1, respectively.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Robbins, S. M. Personal Communication. Baltimore, Md. 2/8/2016. 2. Safari, F., Murata-Kamiya, N., Saito, Y., Hatakeyama, M. Mammalian pragmin regulates Src family kinases via the glu-pro-ile-tyr-ala (EPIYA) motif that is exploited by bacterial effectors. Proc. Nat. Acad. Sci. 108: 14938-14943, 2011. 3. Tanaka, H., Katoh, H., Negishi, M. Pragmin, a novel effector of Rnd2 GTPase, stimulates RhoA activity. J. Biol. Chem 281: 10355-10364, 2006.

Uniprot ID: Q86YV5

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross reactivity with other proteins.

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 200 kDa

Calculated Molecular Weight: 52588 MW

Gene ID: 157285

Protein Name: Inactive tyrosine-protein kinase PRAG1

Gene Full Name: PEAK1 related, kinase-activating pseudokinase 1

Synonyms: PRAG1; SGK223; Inactive tyrosine-protein kinase PRAG1; PEAK1-related kinase-activating pseudokinase 1; Pragmin; Sugen kinase 223; SgK223