Anti-PPM1G Antibody Picoband®

SKU: A06725-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,ICC,IF,IP,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Immunofluorescence, 5 μg/ml, Human
Immunoprecipitation, 0.5-2 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: PPM1G

Specificity:

Background: Protein phosphatase 1G is an enzyme that in humans is encoded by the PPM1G gene. The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase is found to be responsible for the dephosphorylation of Pre-mRNA splicing factors, which is important for the formation of functional spliceosome. Studies of a similar gene in mice suggested a role of this phosphatase in regulating cell cycle progression.

Immunogen: E.coli-derived human PPM1G recombinant protein (Position: E48-Q457).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Gross, M. B. Personal Communication. Baltimore, Md. 9/30/2014. 2. Murray, M. V., Kobayashi, R., Krainer, A. R. The type 2C Ser/Thr phosphatase PP2C-gamma is a pre-mRNA splicing factor. Genes Dev. 13: 87-97, 1999. 3. Travis, S. M., Welsh, M. J. PP2C-gamma: a human protein phosphatase with a unique acidic domain. FEBS Lett. 412: 415-419, 1997.

Uniprot ID: O15355

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 75 kDa

Calculated Molecular Weight: 104553 MW

Gene ID: 63977

Protein Name: Protein phosphatase 1G

Gene Full Name: protein phosphatase, Mg2+/Mn2+ dependent 1G

Synonyms: PP2C gamma; PP2CG; PP2CGAMMA; PPM1C; PPM1G; PPP2CG; Protein phosphatase 1C; Protein phosphatase 1G