Anti-PNKP Antibody Picoband®

SKU: A03566-2

Size: 100 μg

Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,ICC/IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml

Gene Name: PNKP

Specificity:

Background: This locus represents a gene involved in DNA repair. In response to ionizing radiation or oxidative damage, the protein encoded by this locus catalyzes 5' phosphorylation and 3' dephosphorylation of nucleic acids. Mutations at this locus have been associated with microcephaly, seizures, and developmental delay.

Immunogen: E.coli-derived human PNKP recombinant protein (Position: D43-E487). Human PNKP shares 81.8% amino acid (aa) sequence identity with mouse PNKP.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Bras, J., Alonso, I., Barbot, C., Costa, M. M., Darwent, L., Orme, T., Sequeiros, J., Hardy, J., Coutinho, P., Guerreiro, R. Mutations in PNKP cause recessive ataxia with oculomotor apraxia type 4. Am. J. Hum. Genet. 96: 474-479, 2015. 2. Jilani, A., Ramotar, D., Slack, C., Ong, C., Yang, X. M., Scherer, S. W., Lasko, D. D. Molecular cloning of the human gene, PNKP, encoding a polynucleotide kinase 3-prime-phosphatase and evidence for its role in repair of DNA strand breaks caused by oxidative damage. J. Biol. Chem. 274: 24176-24186, 1999. 3. Karimi-Busheri, F., Daly, G., Robins, P., Canas, B., Pappin, D. J. C., Sgouros, J., Miller, G. G., Fakhrai, H., Davis, E. M., Le Beau, M. M., Weinfeld, M. Molecular characterization of a human DNA kinase. J. Biol. Chem. 274: 24187-24194, 1999.

Uniprot ID: Q96T60

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 57 kDa

Calculated Molecular Weight: 57 kDa

Gene ID: 11284

Protein Name: Bifunctional polynucleotide phosphatase/kinase

Gene Full Name: polynucleotide kinase 3'-phosphatase

Synonyms: DEM1; EIEE10; MCSZ; PNK1; Pnkp; PNKP DNA kinase