Anti-PIWIL4/PIWI Antibody Picoband®

SKU: A06797-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,IHC,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: PIWIL4

Specificity:

Background: PIWI-like protein 4 (PIWIL4) is a 97 kDa member of the argonaute family of proteins and the PIWI subfamily. Human PIWIL4 is 852 amino acids (aa) in length and contains one PAZ domain (aa 268-384) and one PIWI domain (aa 546-838). There are three isoforms for human PIWIL4. Isoform 1 is the standard protein. Isoform 2 has a two aa substitution for aa 1-29 in isoform 1 and a deletion of aa 523-852. Isoform 3 has a deletion of aa 1-69 and a deletion of aa 523-852. Human PIWIL4 shares 77% aa sequence identity with mouse and rat PIWIL4. PIWIL4 plays a central role in spermatogenesis.

Immunogen: E.coli-derived human PIWIL4/PIWI recombinant protein (Position: R51-N749).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Carmell, M. A., Girard, A., van de Kant, H. J. G., Bourc'his, D., Bestor, T. H., de Rooij, D. G., Hannon, G. J. MIWI2 is essential for spermatogenesis and repression of transposons in the mouse male germline. Dev. Cell 12: 503-514, 2007. 2. De Fazio, S., Bartonicek, N., Di Giacomo, M., Abreu-Goodger, C., Sankar, A., Funaya, C., Antony, C., Moreira, P. N., Enright, A. J., O'Carroll, D. The endonuclease activity of Mili fuels piRNA amplification that silences LINE1 elements. Nature 480: 259-263, 2011. 3. Sasaki, T., Shiohama, A., Minoshima, S., Shimizu, N. Identification of eight members of the Argonaute family in the human genome. Genomics 82: 323-330, 2003.

Uniprot ID: Q7Z3Z4

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 97 kDa

Calculated Molecular Weight: 104553 MW

Gene ID: 143689

Protein Name: Piwi-like protein 4

Gene Full Name: piwi like RNA-mediated gene silencing 4

Synonyms: PIWIL4; HIWI2; PIWI; Piwi-like protein 4