Anti-PITHD1 Antibody Picoband®

SKU: A15347-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: PITHD1

Specificity:

Background: PITHD1 (PITH (C-terminal proteasome-interacting domain of thioredoxin-like) domain containing 1), also known as HT014, TXNL1CL or C1orf128, is a 211 amino acid nuclear protein containing one PITH domain. PITHD1 ectopic expression is thought to promote megakaryocytic differentiation and increase RUNX1 expression, while knockdown of PITHD1 was observed to have the opposite effect on both differentiation and RUNX1 expression. PITHD1 may regulate RUNX1 expression in two distinct fashions, by increasing transcription activity of proximal promoter and enhancing translational activity of an IRES element. Altered gene expression of the PITHD1 gene has been associated with leukemia development, with significant downregulation of expression observed in leukemic samples. The PITHD1 gene maps to chromosome 1 and is conserved in chimpanzee, Rhesus monkey, canine, bovine, mouse, rat, chicken, zebrafish, fruit fly, mosquito, C. e

Immunogen: E.coli-derived human PITHD1 recombinant protein (Position: C12-S211). Human PITHD1 shares 98% amino acid (aa) sequence identity with mouse PITHD1.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Kondo, H., Matsumura, T., Kaneko, M., Inoue, K., Kosako, H., Ikawa, M., Takahama, Y., Ohigashi, I. PITHD1 is a proteasome-interacting protein essential for male fertilization. J. Biol. Chem. 295: 1658-1672, 2020.

Uniprot ID: Q9GZP4

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity:

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 24 kDa

Calculated Molecular Weight:

Gene ID: 57095

Protein Name: PITH domain-containing protein 1

Gene Full Name: PITH domain containing 1

Synonyms: PITHD1; C1orf128; AD039; HT014; PP603; PITH domain-containing protein 1