Anti-PIAS2 Antibody Picoband®

SKU: A04130-3

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,Flow Cytometry,ELISA

Application Details: Western blot, 0.1-0.25μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human
ELISA, 0.1-0.5μg/ml, -

Gene Name: PIAS2

Specificity: No cross reactivity with other proteins.

Background: E3 SUMO-protein ligase PIAS2 is an enzyme that in humans is encoded by the PIAS2 gene. It is mapped to 18q21.1. This gene encodes a member of the protein inhibitor of activated STAT family, which function as SUMO E3 ligases and play important roles in many cellular processes by mediating the sumoylation of target proteins. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. Isoforms of the encoded protein enhance the sumoylation of specific target proteins including the p53 tumor suppressor protein, c-Jun, and the androgen receptor. A pseudogene of this gene is located on the short arm of chromosome 4. The symbol MIZ1 has also been associated with ZBTB17 which is a different gene located on chromosome 1.

Immunogen: E.coli-derived human PIAS2 recombinant protein (Position: E93-K311).

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Liu, B., Liao, J., Rao, X., Kushner, S. A., Chung, C. D., Chang, D. D., Shuai, K. Inhibition of Stat1-mediated gene activation by PIAS1. Proc. Nat. Acad. Sci. 95: 10626-10631, 1998. 2. Tussie-Luna, M. I., Michel, B., Hakre, S., Roy, A. L. The SUMO ubiquitin-protein isopeptide ligase family member Miz1/PIASx-beta/Siz2 is a transcriptional cofactor for TFII-I. J. Biol. Chem. 277: 43185-43193, 2002. 3. Yang, S.-H., Sharrocks, A. D. PIASx-alpha differentially regulates the amplitudes of transcriptional responses following activation of the ERK and p38 MAPK pathways. Molec. Cell 22: 477-487, 2006.

Uniprot ID: O75928

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 68 kDa

Calculated Molecular Weight: 211344 MW

Gene ID: 9063

Protein Name: E3 SUMO-protein ligase PIAS2

Gene Full Name: protein inhibitor of activated STAT 2

Synonyms: ARIP3; DAB2 interacting protein; DIP; E3 SUMO protein ligase PIAS2; miz; MIZ1; PIAS NY protein; PIAS2; PIASX; PIASX ALPHA; PIASX BETA; SIZ2; ZMIZ4