Anti-PAM Antibody Picoband®

SKU: A00332-2

Size: 100 μg

Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml

Gene Name: PAM

Specificity:

Background: This gene encodes a multifunctional protein. The encoded preproprotein is proteolytically processed to generate the mature enzyme. This enzyme includes two domains with distinct catalytic activities, a peptidylglycine alpha-hydroxylating monooxygenase (PHM) domain and a peptidyl-alpha-hydroxyglycine alpha-amidating lyase (PAL) domain. These catalytic domains work sequentially to catalyze the conversion of neuroendocrine peptides to active alpha-amidated products. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed.

Immunogen: E.coli-derived human PAM recombinant protein (Position: K69-R914). Human PAM shares 97.4% amino acid (aa) sequence identity with mouse PAM.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Eipper, B. A., Green, C. B.-R., Campbell, T. A., Stoffers, D. A., Keutmann, H. T., Mains, R. E., Ouafik, L.'H. Alternative splicing and endoproteolytic processing generate tissue-specific forms of pituitary peptidylglycine alpha-amidating monooxygenase (PAM). J. Biol. Chem. 267: 4008-4015, 1992. 2. Glauder, J., Ragg, H., Rauch, J., Engels, J. W. Human peptidylglycine alpha-amidating monooxygenase: cDNA, cloning and functional expression of a truncated form in COS cells. Biochem. Biophys. Res. Commun. 169: 551-558, 1990. 3. Gross, M. B. Personal Communication. Baltimore, Md. 5/17/2021.

Uniprot ID: P19021

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 120 kDa

Calculated Molecular Weight: 108 kDa

Gene ID: 5066

Protein Name: Peptidyl-glycine alpha-amidating monooxygenase

Gene Full Name: peptidylglycine alpha-amidating monooxygenase

Synonyms: PAL; PAM; Peptidylamidoglycolate lyase; PHM