Anti-Osteoprotegerin/TNFRSF11B Antibody Picoband®

SKU: A00863

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Monkey,Mouse,Rat

Applications: ELISA,Flow Cytometry,IHC,WB

Application Details: Western blot, 0.25-0.5μg/ml, Human, Monkey, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human
ELISA, 0.1-0.5μg/ml, -

Gene Name: TNFRSF11B

Specificity: No cross reactivity with other proteins.

Background: Tumor necrosis factor receptor superfamily member 11B (TNFRSF11B), also known as OPG, is a protein that in humans is encoded by the TNFRSF11B gene. OPG is a cytokine receptor, and a member of the tumor necrosis factor (TNF) receptor superfamily. By analysis of radiation hybrids, TNFRSF11B gene was mapped to chromosome 8q24. OPG is a decoy receptor for the receptor activator of nuclear factor kappa B ligand (RANKL). By binding RANKL, OPG inhibits nuclear kappa B (NF-κB) which is a central and rapid acting transcription factor for immune-related genes, and a key regulator of inflammation, innate immunity, and cell survival and differentiation. OPG binding to RANKL on osteoblast/stromal cells, blocks the RANKL-RANK ligand interaction between osteoblast/stromal cells and ostepclast precursors.

Immunogen: E.coli-derived human Osteoprotegerin/TNFRSF11B recombinant protein (Position: Q247-R296).

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Krakauer T (2008). Nuclear factor-kappa B: fine-tuning a central integrator of diverse biologic stimuli. Int. Rev. Immunol. 27 (5): 286–92. 2. Khosla S (December 2001). Minireview: the OPG/RANKL/RANK system. Endocrinology 142 (12): 5050–5. 3. Schoppet M, Preissner KT, Hofbauer LC (April 2002). RANK ligand and osteoprotegerin: paracrine regulators of bone metabolism and vascular function. Arterioscler. Thromb. Vasc. Biol. 22 (4): 549–53.

Uniprot ID: O00300

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 55 kDa

Calculated Molecular Weight: 34632 MW

Gene ID: 4982

Protein Name: Tumor necrosis factor receptor superfamily member 11B

Gene Full Name: TNF receptor superfamily member 11b

Synonyms: TNF receptor superfamily member 11b; OCIF; OPG; PDB5; TR1; TNFRSF11B