Anti-NUMA/NUMA1 Antibody Picoband®

SKU: A02018-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat,Monkey

Applications: WB,IHC,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat, Monkey
Immunohistochemistry(Paraffin-embedded Section), 1-2 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: NUMA1

Specificity:

Background: Nuclear mitotic apparatus protein 1 is a protein that in humans is encoded by the NUMA1 gene. This gene encodes a large protein that forms a structural component of the nuclear matrix. The encoded protein interacts with microtubules and plays a role in the formation and organization of the mitotic spindle during cell division. Chromosomal translocation of this gene with the RARA (retinoic acid receptor, alpha) gene on chromosome 17 have been detected in patients with acute promyelocytic leukemia. Alternative splicing results in multiple transcript variants.

Immunogen: E.coli-derived human NUMA/NUMA1 recombinant protein (Position: M1-E1954).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Compton, D. A., Szilak, I., Cleveland, D. W. Primary structure of NuMA, an intranuclear protein that defines a novel pathway for segregation of proteins at mitosis. J. Cell Biol. 116: 1395-1408, 1992. 2. Lydersen, B. K., Pettijohn, D. E. Human-specific nuclear protein that associates with the polar region of the mitotic apparatus: distribution in a human/hamster hybrid cell. Cell 22: 489-499, 1980. 3. Merdes, A., Ramyar, K., Vechio, J. D., Cleveland, D. W. A complex of NuMA and cytoplasmic dynein is essential for mitotic spindle assembly. Cell 87: 447-458, 1996.

Uniprot ID: Q14980

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 270 kDa

Calculated Molecular Weight: 37492 MW

Gene ID: 4926

Protein Name: Nuclear mitotic apparatus protein 1

Gene Full Name: nuclear mitotic apparatus protein 1

Synonyms: NMP22; NuMA; NuMA protein; NUMA1; SP H antigen; NMP-22