Anti-NUDT5 Antibody Picoband®

SKU: A07530

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: ELISA,IP,IF IHC,ICC,WB,Flow Cytometry

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Immunoprecipitation, 0.5-2 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: NUDT5

Specificity:

Background: ADP-sugar pyrophosphatase is an enzyme that in humans is encoded by the NUDT5 gene. This gene belongs to the Nudix (nucleoside diphosphate linked moiety X) hydrolase superfamily. The encoded enzyme catalyzes the hydrolysis of modified nucleoside diphosphates, including ADP-ribose (ADPR) and 8-oxoGua-containing 8-oxo-dADP and 8-oxo-dGDP. Protein-bound ADP ribose can be hazardous to the cell because it can modify some amino acid residues, resulting in the inhibition of ATP-activated potassium channels. 8-oxoGua is an oxidized form of guanine that can potentially alter genetic information by pairing with adenine and cytosine in RNA. Presence of 8-oxoGua in RNA results in formation of abnormal proteins due to translational errors.

Immunogen: E.coli-derived human NUDT5 recombinant protein (Position: M1-F219).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Gasmi, L., Cartwright, J. L., McLennan, A. G. Cloning, expression and characterization of YSA1H, a human adenosine 5-prime-diphosphosugar pyrophosphatase possessing a MutT motif. Biochem. J. 344: 331-337, 1999. 2. McLennan, A. G. The MutT family of nucleotide phosphohydrolases in man and human pathogens. Int. J. Molec. Med. 4: 79-89, 1999. 3. Wright, R. H. G., Lioutas, A., Le Dily, F., Soronellas, D., Pohl, A., Bonet, J., Nacht, A. S., Samino, S., Font-Mateu, J., Vicent, G. P., Wierer, M., Trabado, M. A., Schelhorn, C., Carolis, C., Macias, M. J., Yanes, O., Oliva, B., Beato, M. ADP-ribose-derived nuclear ATP synthesis by NUDIX5 is required for chromatin remodeling. Science 352: 1221-1225, 2016.

Uniprot ID: Q9UKK9

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 35 kDa

Calculated Molecular Weight: 24,328 MW

Gene ID: 11164

Protein Name: ADP-sugar pyrophosphatase

Gene Full Name: nudix hydrolase 5

Synonyms: hYSAH1; Nudt5; YSA1; YSA1H