Anti-NUBP1 Antibody Picoband®

SKU: A08538-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,IHC,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: NUBP1

Specificity:

Background: NUBP1 also known as NBP as is expressed in several tissues with the highest being in the lung and testis. NUBP1 appears to interact with actin and the cytoskeleton and is required for centrosome duplication but it is also need for proper synthesis of iron sulfur proteins and the protein also binds ATP. NUBP1 has roles in cell growth, cellular iron homeostasis, centrosome localization, iron-sulfur cluster assembly, and apolipoprotein modification. NUBP1 is localized to the cytoplasm. Interestingly too, recent research indicates that mutations in NUBP1 in mice lead defects in lung development and eye cataracts, many of which are traced back to NUBP1 role in regulating centrosome dynamics and microtubule organization.

Immunogen: E.coli-derived human NUBP1 recombinant protein (Position: M1-Q311).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Hartz, P. A. Personal Communication. Baltimore, Md. 9/11/2012. 2. Nakashima, H., Grahovac, M. J., Mazzarella, R., Fujiwara, H., Kitchen, J. R., Threat, T. A., Ko, M. S. H. Two novel mouse genes--Nubp2, mapped to the t-complex on chromosome 17, and Nubp1, mapped to the chromosome 16--establish a new gene family of nucleotide-binding proteins in eukaryotes. Genomics 60: 152-160, 1999. 3. Shahrestanifar, M., Saha, D. P., Scala, L. A., Basu, A., Howells, R. D. Cloning of a human cDNA encoding a putative nucleotide-binding protein related to Escherichia coli MinD. Gene 147: 281-285, 1994.

Uniprot ID: P53384

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 35 kDa

Calculated Molecular Weight:

Gene ID: 4682

Protein Name: Cytosolic Fe-S cluster assembly factor NUBP1

Gene Full Name: NUBP iron-sulfur cluster assembly factor 1, cytosolic

Synonyms: NBP; NBP 1; NBP1; Nbp35; NUBP1; Nucleotide binding protein 1