Anti-NSUN7 Antibody Picoband®

SKU: A14267

Size: 100 μg

Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml

Gene Name: NSUN7

Specificity:

Background: Predicted to enable RNA binding activity and methyltransferase activity. Predicted to be involved in RNA methylation. Predicted to act upstream of or within flagellated sperm motility and sperm mitochondrion organization.

Immunogen: E.coli-derived human NSUN7 recombinant protein (Position: D83-Q457). Human NSUN7 shares 79.2% amino acid (aa) sequence identity with mouse NSUN7.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Harris, T., Marquez, B., Suarez, S., Schimenti, J. Sperm motility defects and infertility in male mice with a mutation in Nsun7, a member of the Sun domain-containing family of putative RNA methyltransferases. Biol. Reprod. 77: 376-382, 2007. 2. Khosronezhad, N., Hosseinzadeh Colagar, A., Mortazavi, S. M. The Nsun7 (A11337)-deletion mutation, causes reduction of its protein rate and associated with sperm motility defect in infertile men. J. Assist. Reprod. Genet. 32: 807-815, 2015. 3. Ren, H. Y., Zhong, R., Ding, X. P., Chen, Z. Y., Jing, Y. L. Investigation of polymorphisms in exon7 of the NSUN7 gene among Chinese Han men with asthenospermia. Genet. Molec. Res. 14: 9261-9268, 2015.

Uniprot ID: Q8NE18

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 81 kDa

Calculated Molecular Weight: 81 kDa

Gene ID: 79730

Protein Name: Putative methyltransferase NSUN7

Gene Full Name: NOP2/Sun RNA methyltransferase family member 7

Synonyms: NSUN7