Anti-NSUN3 Antibody Picoband®

SKU: A16002

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Monkey

Applications: ELISA,WB,Flow Cytometry

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Monkey
Flow Cytometry (Fixed), 1-3 μg /1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: NSUN3

Specificity:

Background: NSUN3 (NOL1/NOP2/Sun domain family, member 3), also known as MST077, is a 340 amino acid protein that belongs to the methyltransferase superfamily and is thought to function as an S-adenosyl-L-methionine-dependent methyltransferase. The gene encoding NSUN3 maps to human chromosome 3, which houses over 1,100 genes, including a chemokine receptor (CKR) gene cluster and a variety of human cancer-related gene loci. Key tumor suppressing genes on chromosome 3 include those that encode the apoptosis mediator RASSF1, the cell migration regulator HYAL1 and the angiogenesis suppressor SEMA3B. Marfan syndrome, porphyria, von Hippel-Lindau syndrome, osteogenesis imperfecta and Charcot-Marie-Tooth disease are a few of the numerous genetic diseases associated with chromosome 3

Immunogen: E.coli-derived human NSUN3 recombinant protein (Position: K12-E310).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Haag, S., Sloan, K. E., Ranjan, N., Warda, A. S., Kretschmer, J., Blessing, C., Hubner, B., Seikowski, J., Dennerlein, S., Rehling, P., Rodnina, M., Hobartner, C., Bohnsack, M. T. NSUN3 and ABH1 modify the wobble position of mt-tRNA(Met) to expand codon recognition in mitochondrial translation. EMBO J. 35: 2104-2119, 2016. 2. Hartz, P. A. Personal Communication. Baltimore, Md. 5/25/2017. 3. Nakano, S., Suzuki, T., Kawarada, L., Iwata, H., Asano, K., Suzuki, T. NSUN3 methylase initiates 5-formylcytidine biogenesis in human mitochondrial tRNA(Met). Nature Chem. Biol. 12: 546-551, 2016.

Uniprot ID: Q9H649

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 38 kDa

Calculated Molecular Weight: 24145 MW

Gene ID: 63899

Protein Name: tRNA

Gene Full Name: NOP2/Sun RNA methyltransferase 3

Synonyms: NSUN3; MSTP077; UG0651E06; tRNA; cytosine(34)-C(5)-methyltransferase, mitochondrial; NOL1/NOP2/Sun domain familymember 3