Anti-NSUN2 Antibody Picoband®

SKU: A03878-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IF,IHC,ICC,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 1-2 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: NSUN2

Specificity:

Background: NOP2/Sun domain family, member 2 is a protein that in humans is encoded by the NSUN2 gene. This gene encodes a methyltransferase that catalyzes the methylation of cytosine to 5-methylcytosine (m5C) at position 34 of intron-containing tRNA(Leu)(CAA) precursors. This modification is necessary to stabilize the anticodon-codon pairing and correctly translate the mRNA. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.

Immunogen: E.coli-derived human NSUN2 recombinant protein (Position: H146-K654).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Abbasi-Moheb, L., Mertel, S., Gonsior, M., Nouri-Vahid, L., Kahrizi, K., Cirak, S., Wieczorek, D., Motazacker, M. M., Esmaeeli-Nieh, S., Cremer, K., Weissmann, R., Tzschach, A., Garshasbi, M., Abedini, S. S., Najmabadi, H., Ropers, H. H., Sigrist, S. J., Kuss, A. W. Mutations in NSUN2 cause autosomal-recessive intellectual disability. Am. J. Hum. Genet. 90: 847-855, 2012. 2. Blanco, S., Bandiera, R., Popis, M., Hussain, S., Lombard, P., Aleksic, J., Sajini, A., Tanna, H., Cortes-Garrido, R., Gkatza, N., Dietmann, S., Frye, M. Stem cell function and stress response are controlled by protein synthesis. Nature 534: 335-340, 2016. 3. Blanco, S., Dietmann, S., Flores, J. V., Hussain, S., Kutter, C., Humphreys, P., Lukk, M., Lombard, P., Treps, L., Popis, M., Kellner, S., Holter, S. M., and 13 others. l Aberrant methylation of tRNAs links cellular stress to neuro-developmental disorders. EMBO J. 33: 2020-2039, 2014.

Uniprot ID: Q08J23

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 100 kDa

Calculated Molecular Weight: 127459 MW

Gene ID: 54888

Protein Name: RNA cytosine C

Gene Full Name: NOP2/Sun RNA methyltransferase 2

Synonyms: hTrm4; MISU; NSUN2; SAKI; TRM4