Anti-NRF2/NFE2L2 Antibody Picoband®

SKU: A00078-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: ELISA,Flow Cytometry,WB

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg /1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: NFE2L2

Specificity:

Background: Nuclear factor erythroid 2-related factor 2 (NRF2), also known as nuclear factor erythroid-derived 2-like 2, is a transcription factor that in humans is encoded by the NFE2L2 gene. This gene encodes a transcription factor which is a member of a small family of basic leucine zipper (bZIP) proteins. The encoded transcription factor regulates genes which contain antioxidant response elements (ARE) in their promoters; many of these genes encode proteins involved in response to injury and inflammation which includes the production of free radicals. Multiple transcript variants encoding different isoforms have been characterized for this gene.

Immunogen: E.coli-derived human NRF2/NFE2L2 recombinant protein (Position: E67-D601).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Bambouskova, M., Gorvel, L., Lampropoulou, V., Sergushichev, A., Loginicheva, E., Johnson, K., Korenfeld, D., Mathyer, M. E., Kim, H., Huang, L.-H., Duncan, D., Bregman, H., and 19 others. Electrophilic properties of itaconate and derivatives regulate the I-kappa-B-zeta-ATF3 inflammatory axis. Nature 556: 501-504, 2018. 2. Bollong, M. J., Lee, G., Coukos, J. S., Yun, H., Zambaldo, C., Chang, J. W., Chin, E. N., Ahmad, I., Chatterjee, A. K., Lairson, L. L., Schultz, P. G., Moellering, R. E. A metabolite-derived protein modification integrates glycolysis with KEAP1-NRF2 signalling. Nature 562: 600-604, 2018. 3. Chan, J. Y., Cheung, M.-C., Moi, P., Chan, K., Kan, Y. W. Chromosomal localization of the human NF-E2 family of bZIP transcription factors by fluorescence in situ hybridization. Hum. Genet. 95: 265-269, 1995

Uniprot ID: Q16236

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity:

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 100-110 kDa

Calculated Molecular Weight: 68 kDa

Gene ID: 4780

Protein Name: Nuclear factor erythroid 2-related factor 2

Gene Full Name: NFE2 like bZIP transcription factor 2

Synonyms: HEBP1; NF E2 related factor 2; NFE2 related factor 2; NFE2L2; NRF2; NRF2; NFE2L2