Anti-NR5A2/LRH1 Antibody Picoband®

SKU: PB9298

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB

Application Details: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Gene Name: NR5A2

Specificity: No cross reactivity with other proteins.

Background: NR5A2 (nuclear receptor subfamily 5, group A, member 2) also known as liver receptor homolog-1 (LRH-1) is a protein that in humans is encoded by the NR5A2 gene. LRH-1 is a member of the nuclear receptor family of intracellulartranscription factors. LRH-1 plays a critical role in the regulation of development,cholesterol transport, bile acid homeostasis andsteroidogenesis. LRH-1 is important for maintaining pluripotence of stem cells during embryonic development. Liver receptor homolog-1 has been shown to interact with the small heterodimer partner.

Immunogen: E.coli-derived human NR5A2 recombinant protein (Position: K44-R237). Human NR5A2 shares 95% amino acid (aa) sequence identity with both mouse and rat NR5A2.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Goodwin, B., Jones, S. A., Price, R. R., Watson, M. A., McKee, D. D., Moore, L. B., Galardi, C., Wilson, J. G., Lewis, M. C., Roth, M. E., Maloney, P. R., Willson, T. M., Kliewer, S. A. A regulatory cascade of the nuclear receptors FXR, SHP-1, and LRH-1 represses bile acid biosynthesis. Molec. Cell 6: 517-526, 2000. 2. Gu, P.; Goodwin, B.; Chung, A. C. -K.; Xu, X.; Wheeler, D. A.; Price, R. R.; Galardi, C.; Peng, L.; Latour, A. M.; Koller, B. H.; Gossen, J.; Kliewer, S. A.; Cooney, A. J. (2005). Orphan Nuclear Receptor LRH-1 is Required to Maintain Oct4 Expression at the Epiblast Stage of Embryonic Development. Molecular and Cellular Biology 25(9): 3492–3505.

Uniprot ID: O00482

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 70 kDa

Calculated Molecular Weight: 61 kDa

Gene ID: 2494

Protein Name: Nuclear receptor subfamily 5 group A member 2

Gene Full Name: nuclear receptor subfamily 5 group A member 2

Synonyms: B1 binding factor; B1F; B1F2; CPF; CYP7A promoter binding factor; FTF; FTZ F1; FTZ F1beta; hB1F; hB1F 2; Liver receptor homolog 1; LRH 1; NR5A2