Anti-non-muscle Myosin IIB/MYH10 Antibody Picoband®

SKU: A02681-1

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,ICC,IF,Flow Cytometry

Application Details: Western blot, 0.1-0.25μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat
Immunocytochemistry/Immunofluorescence, 4μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human, Mouse, Rat

Gene Name: MYH10

Specificity:

Background: This gene encodes a member of the myosin superfamily. The protein represents a conventional non-muscle myosin; it should not be confused with the unconventional myosin-10 (MYO10). Myosins are actin-dependent motor proteins with diverse functions including regulation of cytokinesis, cell motility, and cell polarity. Mutations in this gene have been associated with May-Hegglin anomaly and developmental defects in brain and heart. Multiple transcript variants encoding different isoforms have been found for this gene.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human non-muscle Myosin IIB/MYH10, identical to the related mouse and rat sequences.

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Ma, X., Adelstein, R. S. A point mutation in Myh10 causes major defects in heart development and body wall closure. Circ. Cardiovasc. Genet. 7: 257-265, 2014. Note: Erratum: Circ. Cardiovasc. Genet. 7: 570 only, 2014. 2. Tuzovic, L., Yu, L., Zeng, W., Li, X., Lu, H., Lu, H.-M., Gonzalez, K., Chung, W. K. A human de novo mutation in MYH10 phenocopies the loss of function mutation in mice. Rare Dis. 1: e26144, 2013. Note: Electronic Article.

Uniprot ID: P35580

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 229 kDa

Calculated Molecular Weight: 33310 MW

Gene ID: 4628

Protein Name: Myosin-10

Gene Full Name: myosin heavy chain 10

Synonyms: MYH10; Myosin 10; Myosin heavy chain 10