Anti-Ninein/NIN Antibody Picoband®

SKU: A03001-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: NIN

Specificity:

Background: Ninein is a protein that in humans is encoded by the NIN gene. This gene encodes one of the proteins important for centrosomal function. This protein is important for positioning and anchoring the microtubules minus-ends in epithelial cells. Localization of this protein to the centrosome requires three leucine zippers in the central coiled-coil domain. Multiple alternatively spliced transcript variants that encode different isoforms have been reported.

Immunogen: E.coli-derived human Ninein/NIN recombinant protein (Position: Q1023-H2090).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Chen, C.-H., Howng, S.-L., Cheng, T.-S., Chou, M.-H., Huang, C.-Y., Hong, Y.-R. Molecular characterization of human ninein protein: two distinct subdomains required for centrosomal targeting and regulating signals in cell cycle. Biochem. Biophys. Res. Commun. 308: 975-983, 2003. 2. Chou, C. H., Loh, J. K., Yang, M. C., Lin, C. C., Hong, M. C., Cho, C. L., Chou, A. K., Wang, C. H., Lieu, A. S., Howng, S. L., Hsu, C. M., Hong, Y. R. AIBp regulates mitotic entry and mitotic spindle assembly by controlling activation of both Aurora-A and Plk1. Cell Cycle 14: 2764-2776, 2015. 3. Dauber, A., LaFranchi, S. H., Maliga, Z., Lui, J. C., Moon, J. E., McDeed, C., Henke, K., Zonana, J., Kingman, G. A., Pers, T. H., Baron, J., Rosenfeld, R. G., Hirschhorn, J. N., Harris, M. P., Hwa, V. Novel microcephalic primordial dwarfism disorder associated with variants in the centrosomal protein ninein. J. Clin. Endocr. Metab. 97: E2140-E2151, 2012. Note: Electronic Article.

Uniprot ID: Q8N4C6

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 260 kDa

Calculated Molecular Weight:

Gene ID: 51199

Protein Name: Ninein

Gene Full Name: ninein

Synonyms: GSK3B interacting protein; hNinein; KIAA1565; NIN; Ninein