Anti-NGEF Antibody Picoband®

SKU: A08935-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: ELISA,Flow Cytometry,WB

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg /1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: NGEF

Specificity:

Background: Enables guanyl-nucleotide exchange factor activity. Predicted to be involved in several processes, including activation of GTPase activity; ephrin receptor signaling pathway; and negative regulation of dendritic spine morphogenesis. Predicted to be located in cytosol. Predicted to be active in glutamatergic synapse.

Immunogen: E.coli-derived human NGEF recombinant protein (Position: E82-Q627). Human NGEF shares 91.1% and 90.9% amino acid (aa) sequence identity with mouse and rat NGEF, respectively.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Frank, C. A., Pielage, J., Davis, G. W. A presynaptic homeostatic signaling system composed of the Eph receptor, Ephexin, Cdc42, and Cav2.1 calcium channels. Neuron 61: 556-569, 2009. 2. Rodrigues, N. R., Theodosiou, A. M., Nesbit, M. A., Campbell, L., Tandle, A. T., Saranath, D., Davies, K. E. Characterization of Ngef, a novel member of the Dbl family of genes expressed predominantly in the caudate nucleus. Genomics 65: 53-61, 2000. 3. Shamah, S. M., Lin, M. Z., Goldberg, J. L., Estrach, S., Sahin, M., Hu, L., Bazalakova, M., Neve, R. L., Corfas, G., Debant, A., Greenberg, M. E. EphA receptors regulate growth cone dynamics through the novel guanine nucleotide exchange factor ephexin. Cell 105: 233-244, 2001.

Uniprot ID: Q8N5V2

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity:

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 71 kDa

Calculated Molecular Weight: 82,71 kDa

Gene ID: 25791

Protein Name: Ephexin-1

Gene Full Name: neuronal guanine nucleotide exchange factor

Synonyms: NGEF; Ephexin-1; Eph-interacting exchange protein; Neuronal guanine nucleotide exchange factor