Anti-Neurokinin 1 Receptor/TACR1 Antibody Picoband®

SKU: PA1684

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB

Application Details: Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Gene Name: TACR1

Specificity: No cross reactivity with other proteins.

Background: The tachykinin receptor 1 (TACR1) also known as neurokinin 1 receptor (NK1R) or substance P receptor (SPR) is a G protein coupled receptor found in the central nervous system and peripheral nervous system. The endogenous ligand for this receptor is Substance P, although it has some affinity for other tachykinins. The protein is the product of the TACR1 gene. Tachykinin receptor 1 consists of 407 amino acid residues, and it has a molecular weight of 58.000. Tachykinin receptor 1, as well as the other tachykinin receptors, is made of seven hydrophobic transmembrane (TM) domains with three extracellular and three intracellular loops, an amino-terminus and a cytoplasmic carboxy-terminus. The loops have functional sites, including two cysteines amino acids for a disulfide bridge, Asp-Arg-Tyr, which is responsible for association with arrestin and, Lys/Arg-Lys/Arg-X-X-Lys/Arg, which interacts with G-proteins.The tachykinin receptor 1 can be found in both the central and peripheral nervous system. It is present in neurons, brainstem, vascular endothelial cells, muscle, gastrointestinal tracts, genitourinary tract, pulmonary tissue, thyroid gland and different types of immune cells. The binding of SP to the tachykinin receptor 1 has been associated with the transmission of stress signals and pain, the contraction of smooth muscles and inflammation. Tachykinin receptor 1 antagonists have also been studied in migraine, emesis and psychiatric disorders. In fact, aprepitant has been proved effective in a number of pathophysiological models of anxiety and depression. Other diseases in which the tachykinin receptor 1 system is involved include asthma, rheumatoid arthritis and gastrointestinal disorders.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human TACR1, identical to the related rat and mouse sequences.

Clonality: Polyclonal

Contents: Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Castagliuolo, I., Riegler, M., Pasha, A., Nikulasson, S., Lu, B., Gerard, C., Gerard, N. P., Pothoulakis, C.Neurokinin-1 (NK-1) receptor is required in Clostridium difficile-induced enteritis.J. Clin. Invest. 101: 1547-1550, 1998. 2. George, D. T., Gilman, J., Hersh, J., Thorsell, A., Herion, D., Geyer, C., Peng, X., Kielbasa, W., Rawlings, R., Brandt, J. E., Gehlert, D. R., Tauscher, J. T., Hunt, S. P., Hommer, D., Heilig, M.Neurokinin 1 receptor antagonism as a possible therapy for alcoholism.Science 319: 1536-1539, 2008. 3. Lai, J.-P., Ho, W. Z., Kilpatrick, L. E., Wang, X., Tuluc, F., Korchak, H. M., Douglas, S. D.Full-length and truncated neurokinin-1 receptor expression and function during monocyte/macrophage differentiation.Proc. Nat. Acad. Sci. 103: 7771-7776, 2006.

Uniprot ID: P25103

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 55 kDa

Calculated Molecular Weight: 46251 MW

Gene ID: 6869

Protein Name: Substance-P receptor

Gene Full Name: tachykinin receptor 1

Synonyms: Neurokinin-1 receptor; NK 1 receptor; NK 1R; NK1R; NK-1R; NKIR; SPR; Substance P receptor; TAC1R; tachykinin receptor 1; TACR1