Anti-Neurofibromin/NF1 Antibody Picoband® (monoclonal, 4F8B7)

SKU: M00043-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,ICC,IF,Flow Cytometry

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Gene Name: NF1

Specificity:

Background: Neurofibromin 1 (NF1) is a gene in humans that is located on chromosome 17. This gene product appears to function as a negative regulator of the ras signal transduction pathway. Mutations in this gene have been linked to neurofibromatosis type 1, juvenile myelomonocytic leukemia and Watson syndrome. The mRNA for this gene is subject to RNA editing (CGA>UGA->Arg1306Term) resulting in premature translation termination. Alternatively spliced transcript variants encoding different isoforms have also been described for this gene.

Immunogen: E.coli-derived human Neurofibromin/NF1 recombinant protein (Position: R160-Q270).

Clonality: Monoclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Scheffzek K, Welti S (2012). Neurofibromin: Protein Domains and Functional Characteristics. In Upadhyaya M, Cooper D (eds.). Neurofibromatosis Type 1. Berlin, Heidelberg: Springer. pp. 305–326. 2. Trovó-Marqui AB, Tajara EH (July 2006). Neurofibromin: a general outlook. Clinical Genetics. 70 (1): 1–13. 3. Peltonen S, Pöyhönen M (2012). Clinical Diagnosis and Atypical Forms of NF1. In Upadhyaya M, Cooper D (eds.). Neurofibromatosis Type 1. Berlin, Heidelberg: Springer. pp. 17–30.

Uniprot ID: P21359

Host: Mouse

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Mouse IgG2a

Phospho_site:

Clone Number: Clone: 4F8B7

Observed Molecular Weight: 319 kDa

Calculated Molecular Weight: 14377 MW

Gene ID: 4763

Protein Name: Neurofibromin

Gene Full Name: neurofibromin 1

Synonyms: Neurofibromin; neurofibromin 1; NF1; NFNS; VRNF; WSS