Anti-NEK5 Antibody Picoband®

SKU: A14002-3

Size: 100 μg

Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Mouse,Rat

Applications: WB,IHC,ICC/IF,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Mouse
ELISA, 0.1-0.5 μg/ml

Gene Name: NEK5

Specificity:

Background: Enables protein kinase activity. Acts upstream of or within positive regulation of striated muscle cell differentiation. Predicted to be located in sperm flagellum. Is expressed in choroid invagination; diencephalon roof plate; medulla oblongata part of 4th ventricle choroid plexus; and metencephalon part of 4th ventricle choroid plexus. Orthologous to human NEK5 (NIMA related kinase 5).

Immunogen: E.coli-derived mouse NEK5 recombinant protein (Position: K22-Q627). Mouse NEK5 shares 80.8% amino acid (aa) sequence identity with human NEK5.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Gross, M. B. Personal Communication. Baltimore, Md. 1/8/2016. 2. Melo Hanchuk, T. D., Papa, P. F., La Guardia, P. G., Vercesi, A. E., Kobarg, J. Nek5 interacts with mitochondrial proteins and interferes negatively in mitochondrial mediated cell death and respiration. Cell. Signal. 27: 1168-1177, 2015. 3. Oakley, B. R., Morris, N. R. A mutation in Aspergillus nidulans that blocks the transition from interphase to prophase. J. Cell. Biol. 96: 1155-1158, 1983.

Uniprot ID: Q7TSC3

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 80 kDa

Calculated Molecular Weight: 81 kDa

Gene ID: 330721

Protein Name: Serine/threonine-protein kinase Nek5

Gene Full Name: NIMA (never in mitosis gene a)-related expressed kinase 5

Synonyms: Nek5; Serine/threonine-protein kinase Nek5; EC 2.7.11.1; Never in mitosis A-related kinase 5; NimA-related protein kinase 5