Anti-Nedd4 Antibody Picoband®

SKU: A00984-4

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Mouse,Rat

Applications: ELISA,Flow Cytometry,IF,IHC,WB

Application Details: Western blot, 0.25-0.5 μg/ml, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Mouse, Rat
Immunofluorescence, 5 μg/ml, Mouse
Flow Cytometry (Fixed), 1-3 μg/1x10^6 cells, Mouse
ELISA, 0.1-0.5 μg/ml, -

Gene Name: Nedd4

Specificity:

Background: E3 ubiquitin-protein ligase NEDD4, also known as neural precursor cell expressed developmentally down-regulated protein 4 (NEDD4), is an enzyme that in humans is encoded by the NEDD4 gene. This gene is the founding member of the NEDD4 family of HECT ubiquitin ligases that function in the ubiquitin proteasome system of protein degradation. The encoded protein contains an N-terminal calcium and phospholipid binding C2 domain followed by multiple tryptophan-rich WW domains and, a C-terminal HECT ubiquitin ligase catalytic domain. It plays critical role in the regulation of a number of membrane receptors, endocytic machinery components and the tumor suppressor PTEN.

Immunogen: E.coli-derived mouse Nedd4 recombinant protein (Position: A7-L655).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Kumar S, Harvey KF, Kinoshita M, Copeland NG, Noda M, Jenkins NA (May 1997). cDNA cloning, expression analysis, and mapping of the mouse Nedd4 gene. Genomics. 40 (3): 435–43. 2. Imhof MO, McDonnell DP (Jul 1996). Yeast RSP5 and its human homolog hRPF1 potentiate hormone-dependent activation of transcription by human progesterone and glucocorticoid receptors. Mol Cell Biol. 16 (6): 2594–605.

Uniprot ID: P46935

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 115 kDa

Calculated Molecular Weight:

Gene ID: 17999

Protein Name: E3 ubiquitin-protein ligase NEDD4

Gene Full Name: neural precursor cell expressed, developmentally down-regulated 4

Synonyms: KIAA0093; NEDD 4; NEDD4; NEDD4 1; RPF1