Anti-NDUFA10 Antibody Picoband®

SKU: A10517-1

Size: 100 μg

Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Rat

Applications: WB,IHC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human, Rat
Immunofluorescence, 5 μg/ml, Human, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml

Gene Name: NDUFA10

Specificity:

Background: The protein encoded by this gene is a component of 42 kDa complex I, the first enzyme complex in the electron transport chain of mitochondria. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. A mutation in this gene was found in an individual with Leigh syndrome.

Immunogen: E.coli-derived human NDUFA10 recombinant protein (Position: H85-R332). Human NDUFA10shares 91.7% and 90.1% amino acid (aa) sequence identity with mouse and rat NDUFA10, respectively.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Amberger, J. S. Personal Communication. Baltimore, Md. 6/11/2008. 2. Baens, M., Chaffanet, M., Cassiman, J.-J., van den Berghe, H., Marynen, P. Construction and evaluation of a hncDNA library of human 12p transcribed sequences derived from a somatic cell hybrid. Genomics 16: 214-218, 1993. 3. Hoefs, S. J. G., van Spronsen, F. J., Lenssen, E. W. H., Nijtmans, L. G., Rodenburg, R. J., Smeitink, J. A. M., van den Heuvel, L. P. NDUFA10 mutations cause complex I deficiency in a patient with Leigh disease. Europ. J. Hum. Genet. 19: 270-274, 2011.

Uniprot ID: O95299

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 41 kDa

Calculated Molecular Weight: 41 kDa

Gene ID: 4705

Protein Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial

Gene Full Name: NADH:ubiquinone oxidoreductase subunit A10

Synonyms: NDUFA10; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial; Complex I-42kD; CI-42kD; NADH-ubiquinone oxidoreductase 42 kDa subunit