Anti-NCOA5 Antibody Picoband®

SKU: A07810-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: NCOA5

Specificity:

Background: Nuclear receptor coactivator 5 (NCOA5), also known as coactivator independent of AF-2 function (CIA), is a protein that in humans is encoded by the NCOA5 gene. This gene encodes a coregulator for the alpha and beta estrogen receptors and the orphan nuclear receptor NR1D2. The protein localizes to the nucleus, and is thought to have both coactivator and corepressor functions. Its interaction with nuclear receptors is independent of the AF2 domain on the receptors, which is known to regulate interaction with other coreceptors. Several alternatively spliced transcript variants have been found for this gene.

Immunogen: E.coli-derived human NCOA5 recombinant protein (Position: R191-Q560).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Gao, S., Li, A., Liu, F., Chen, F., Williams, M., zhang, C., Kelley, Z., Wu, C.-L., Luo, R., Xiao, H. NCOA5 haploinsufficiency results in glucose intolerance and subsequent hepatocellular carcinoma. Cancer Cell 24: 725-737, 2013. 2. Sauve, F., McBroom, L. D. B., Gallant, J., Moraitis, A. N., Labrie, F., Giguere, V. CIA, a novel estrogen receptor coactivator with a bifunctional nuclear receptor interacting determinant. Molec. Cell. Biol. 21: 343-353, 2001.

Uniprot ID: Q9HCD5

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 66 kDa

Calculated Molecular Weight: 49229 MW

Gene ID: 57727

Protein Name: Nuclear receptor coactivator 5

Gene Full Name: nuclear receptor coactivator 5

Synonyms: NCOA5; KIAA1637; Nuclear receptor coactivator 5; NCoA-5; Coactivator independent of AF-2; CIA