Anti-Mucin Gastric MUC5AC Antibody (Monoclonal, 45M1)

SKU: MA1061

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Rabbit,Rat

Applications: IHC,ICC,WB

Application Details: Immunohistochemistry (Paraffin-embedded Section), 2-4μg/ml, Human, rabbit, rat
Immunocytochemistry , 1μg/ml, Human, rabbit, rat, -
Western blot, 1-2μg/ml, Human, rabbit, rat

Gene Name: MUC5B

Specificity: No cross reactivity with other proteins.

Background: Mucin 5AC  (Muc5AC) is a protein that in humans is encoded by the MUC5AC gene. Using real-time RT-PCR, Moehle et al.  (2006) found that MUC5AC was highly expressed in adult trachea and stomach. Expression was lower in adult lung and weak in fetal lung, and no expression was detected in other tissues. This gene has been linked to mucus hypersecretion in the pulmonary tracts and associated to chronic obstructive pulmonary disease. Moreover, Muc5ac is identified as a  and critical mediator of resistance during intestinal nematode infection.

Immunogen: Mucin from human ovarian cyst fluid.

Clonality: Monoclonal

Contents: Mouse IgG in stabilizing components, 1.2% sodium acetate and 0.01mg NaN3. *This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Purification: Ascites

Reconstitution: Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Reference: 1. Hasnain, S. Z., Evans, C. M., Roy, M., Gallagher, A. L., Kindrachuk, K. N., Barron, L., Dickey, B. F., Wilson, M. S., Wynn, T. A., Grencis, R. K., Thornton, D. J. Muc5ac: a critical component mediating the rejection of enteric nematodes. J. Exp. Med. 208: 893-900, 2011. 2. Moehle, C., Ackermann, N., Langmann, T., Aslanidis, C., Kel, A., Kel-Margoulis, O., Schmitz-Madry, A., Zahn, A., Stremmel, W., Schmitz, G. Aberrant intestinal expression and allelic variants of mucin genes associated with inflammatory bowel disease. J. Molec. Med. 84: 1055-1066, 2006.

Uniprot ID: P98088

Host: Mouse

Concentration: Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Mouse IgG1

Phospho_site:

Clone Number: Clone: 45M1

Observed Molecular Weight: 200 kDa

Calculated Molecular Weight: 585570 MW

Gene ID: Human(4586), Mouse(17833), Rat(682837)

Protein Name: Mucin-5AC

Gene Full Name: mucin 5AC, oligomeric mucus/gel-forming

Synonyms: mucin 5AC; oligomeric mucus/gel-forming; MUC5; TBM; leB; mucin; MUC5AC