Anti-MTCL1 Antibody Picoband®

SKU: A10954-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: MTCL1

Specificity:

Background: MTCL1 (microtubule cross-linking factor 1), also known as CCDC165 (Coiledcoil domain-containing protein 165), SOGA2 or MTCL1, is a 1,905 amino acid protein that localizes to the cell membrane, cytoplasm and cytoskeleton. MTCL1 is a microtubule-associated factor that plays a role in regulating polarization and microtubule dynamics as well as the development and maintenance of non-centrosomal microtubule bundles. MTCL1 is encoded by a gene that maps to chromosome 18 and is expressed as four isoforms due to alternative splicing events. Chromosome 18 encodes over 300 genes and contains about 76 million bases. Trisomy 18, or Edwards syndrome, is the second most common trisomy after Downs syndrome. Symptoms of Edwards syndrome include low birth weight, a variety of physical development defects, heart deformations and breathing difficulty. Translocation between chromosome 18 and 14 is the most common translocation in cancers, and occurs in follicular lymphomas. Niemann-Pick disease, hereditary hemorrhagic telangiectasia and erythropoietic protoporphyria are associated with chromosome 18. The TGFβ modulators, Smad2, Smad4 and Smad7 are encoded by chromosome 18.

Immunogen: E.coli-derived human MTCL1 recombinant protein (Position: K884-D1522).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Hartz, P. A. Personal Communication. Baltimore, Md. 4/28/2014. 2. Nagase, T., Ishikawa, K., Suyama, M., Kikuno, R., Miyajima, N., Tanaka, A., Kotani, H., Nomura, N., Ohara, O. Prediction of the coding sequences of unidentified human genes. XI. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro. DNA Res. 5: 277-286, 1998. 3. Sato, Y., Akitsu, M., Amano, Y., Yamashita, K., Ide, M., Shimada, K., Yamashita, A., Hirano, H., Arakawa, N., Maki, T., Hayashi, I., Ohno, S., Suzuki, A. The novel PAR-1-binding protein MTCL1 has crucial roles in organizing microtubules in polarizing epithelial cells. J. Cell Sci. 126: 4671-4683, 2013.

Uniprot ID: Q9Y4B5

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 270-300 kDa

Calculated Molecular Weight:

Gene ID: 23255

Protein Name: Microtubule cross-linking factor 1

Gene Full Name: microtubule crosslinking factor 1

Synonyms: MTCL1; CCDC165; KIAA0802; SOGA2