Anti-MRP8/S100A8 Antibody Picoband®

SKU: PB9742

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Mouse,Rat

Applications: Flow Cytometry,IF,IHC,ICC,WB

Application Details: Western blot, 0.1-0.5μg/ml, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Mouse
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Mouse

Gene Name: S100a8

Specificity: No cross reactivity with recombinant human S100A8 is observed.

Background: S100 calcium-binding protein A8 (S100A8) is a protein that in humans is encoded by the S100A8 gene. It is also known as calgranulin A. The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine. Altered expression of this protein is associated with the disease cystic fibrosis.

Immunogen: E.coli-derived mouse S100A8 recombinant protein (Position: P2-E89). Mouse S100A8 shares 58.6% and 80.5% amino acid (aa) sequence identity with human and rat S100A8, respectively.

Clonality: Polyclonal

Contents: Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Entrez Gene: S100A8 S100 calcium binding protein A8. 2. Kerkhoff C, Klempt M, Sorg C (1999). Novel insights into structure and function of MRP8 (S100A8) and MRP14 (S100A9).. Biochim. Biophys. Acta 1448 (2): 200–11. 3. Schäfer T, Sachse GE, Gassen HG (1991). The calcium-binding protein MRP-8 is produced by human pulmonary tumor cells.. Biol. Chem. Hoppe-Seyler 372 (1): 1–4.

Uniprot ID: P27005

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with recombinant human S100A8 is observed.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 11 kDa

Calculated Molecular Weight: 10295 MW

Gene ID: 20201

Protein Name: Protein S100-A8

Gene Full Name: S100 calcium binding protein A8 (calgranulin A)

Synonyms: 60B8AG; CAGA; Calgranulin A; Calprotectin L1L subunit; CFAG; CGLA; CP 10; Cystic fibrosis antigen; L1Ag; MA387; MIF; MRP 8; MRP8; NIF; P8; Protein S100 A8; S100A8; Urinary stone protein band A