Anti-MIRO1/RHOT1 Antibody Picoband®

SKU: A05928-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,ICC,IF,ELISA

Application Details: Western blot, 0.1-0.25 μg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: RHOT1

Specificity:

Background: Mitochondrial Rho GTPase 1 (MIRO1) is an enzyme that in humans is encoded by the RHOT1 gene on chromosome 17. The Ras homolog family member T1 (RHOT) is an atypical Rho Ca2+-binding GTPase that localizes to the mitochondria. RHOT1, the related protein RHOT2, the adaptor protein Milton, and the PTEN induced putative kinase 1 (PINK1), form a complex that is involved in axonal transport of mitochondria. Both PINK1 and Parkin target RHOT1 for phosphorylation and degradation, causing the arrest of mitochondrial motility.

Immunogen: E.coli-derived human MIRO1/RHOT1 recombinant protein (Position: Q63-A596).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Fransson, A., Ruusala, A., Aspenstrom, P. Atypical Rho GTPases have roles in mitochondrial homeostasis and apoptosis. J. Biol. Chem. 278: 6495-6502, 2003. 2. Hartz, P. A. Personal Communication. Baltimore, Md. 4/11/2011.

Uniprot ID: Q8IXI2

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 71 kDa

Calculated Molecular Weight:

Gene ID: 55288

Protein Name: Mitochondrial Rho GTPase 1

Gene Full Name: ras homolog family member T1

Synonyms: RHOT1; ARHT1; Mitochondrial Rho GTPase 1; MIRO-1; hMiro-1; EC 3.6.5.-; Rac-GTP-binding protein-like protein; Ras homolog gene family member T1