Anti-MICAL3 Antibody Picoband®

SKU: A08089

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: MICAL3

Specificity:

Background: Microtubule-associated monoxygenase, calponin and LIM domain containing 3, also known as MICAL3, is a human gene. MICAL3 (microtubule associated monoxygenase, calponin and LIM domain containing 3), also known as KIAA1364, is a 976 amino acid protein that localizes to both the cytoplasm and the cytoskeleton and contains one LIM zinc-binding domain and one calponin-homology domain. Expressed ubiquitously, MICAL3 uses FAD as a cofactor to interact with Rab 1B and, via this interaction, is thought to play a role in spermatid development. MICAL3 exists as two alternatively spliced isoforms that are encoded by a gene which maps to human chromosome 22q11.21. Chromosome 22 houses over 500 genes, some of which are involved in Phelan-McDermid syndrome, schizophrenia and Neurofibromatosis type 2.

Immunogen: E.coli-derived human MICAL3 recombinant protein (Position: K458-L1592). Human MICAL3 shares 81.9% amino acid (aa) sequence identity with mouse MICAL3.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Hartz, P. A. Personal Communication. Baltimore, Md. 1/28/2015. 2. Li, C., Gonsalves, C. S., Eiymo Mwa Mpollo, M.-S., Malik, P., Tahara, S. M., Kalra, V. K. MicroRNA 648 targets ET-1 mRNA and is cotranscriptionally regulated with MICAL3 by PAX5. Molec. Cell. Biol. 35: 514-528, 2015. 3. Nagase, T., Kikuno, R., Ishikawa, K., Hirosawa, M., Ohara, O. Prediction of the coding sequences of unidentified human genes. XVI. The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro. DNA Res. 7: 65-73, 2000.

Uniprot ID: Q7RTP6

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity:

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 224-280 kDa

Calculated Molecular Weight:

Gene ID: 57553

Protein Name: [F-actin]-monooxygenase MICAL3

Gene Full Name: microtubule associated monooxygenase, calponin and LIM domain containing 3

Synonyms: MICAL3; KIAA0819; KIAA1364; [F-actin]-monooxygenase MICAL3; Molecule interacting with CasL protein 3; MICAL-3