Anti-Metabotropic Glutamate Receptor 5/GRM5 Antibody Picoband®

SKU: A01338-3

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Rat

Applications: WB,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Rat
ELISA, 0.1-0.5 μg/ml, -

Gene Name: GRM5

Specificity:

Background: Metabotropic glutamate receptor 5?is a?G protein-coupled receptor?that in humans is encoded by the?GRM5 gene. It is mapped to 11q14.2-q14.3. This gene encodes a member of the G-protein coupled receptor 3 protein family. The encoded protein is a metabatropic glutamate receptor, whose signaling activates a phosphatidylinositol-calcium second messenger system. This protein may be involved in the regulation of neural network activity and synaptic plasticity. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. A pseudogene of this gene has been defined on chromosome 11. Alternative splicing results in multiple transcript variants.

Immunogen: E.coli-derived human Metabotropic Glutamate Receptor 5/GRM5 recombinant protein (Position: K443-S1050).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Minakami R, Katsuki F, Yamamoto T, Nakamura K, Sugiyama H (March 1994). Molecular cloning and the functional expression of two isoforms of human metabotropic glutamate receptor subtype 5. Biochemical and Biophysical Research Communications. 199 (3): 1136–43. 2. Chen Y, Goudet C, Pin JP, Conn PJ (March 2008). N-{4-Chloro-2-[(1,3-dioxo-1,3-dihydro-2H-isoindol-2-yl)methyl]phenyl}-2-hydroxybenzamide (CPPHA) acts through a novel site as a positive allosteric modulator of group 1 metabotropic glutamate receptors. Molecular Pharmacology. 73 (3): 909–18. 3. Watkins JC, Jane DE (January 2006). The glutamate story. British Journal of Pharmacology. 147 Suppl 1 (Suppl 1): S100–8. doi:10.1038/sj.bjp.0706444.

Uniprot ID: P41594

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 178 kDa

Calculated Molecular Weight:

Gene ID: 2915

Protein Name: Metabotropic glutamate receptor 5

Gene Full Name: glutamate metabotropic receptor 5

Synonyms: glutamate metabotropic receptor 5; GPRC1E; MGLUR5; PPP1R86; mGlu5; GRM5