Anti-MEIOB Antibody Picoband®

SKU: A13211-2

Size: 100 μg

Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,ICC/IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, Human

Gene Name: MEIOB

Specificity:

Background: Predicted to enable chromatin binding activity; single-stranded DNA 3'-5' exodeoxyribonuclease activity; and single-stranded DNA binding activity. Predicted to be involved in double-strand break repair via homologous recombination; fertilization; and meiotic nuclear division. Predicted to be located in cytoplasm. Implicated in spermatogenic failure 22.

Immunogen: E.coli-derived human MEIOB recombinant protein (Position: I140-V442). Human MEIOB shares 77.9% and 76.1% amino acid (aa) sequence identity with mouse and rat MEIOB, respectively.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Caburet, S., Todeschini, A.-L., Petrillo, C., Martini, E., Farran, N. D., Legois, B., Livera, G., Younis, J. S., Shalev, S., Veitia, R. A. A truncating MEIOB mutation responsible for familial primary ovarian insufficiency abolishes its interaction with its partner SPATA22 and their recruitment to DNA double-strand breaks. EBioMedicine 42: 524-531, 2019. 2. Gershoni, M., Hauser, R., Yogev, L., Lehavi, O., Azem, F., Yavetz, H., Pietrokovski, S., Kleiman, S. E. A familial study of azoospermic men identifies three novel causative mutations in three new human azoospermia genes. Genet. Med. 19: 998-1006, 2017. 3. Luo, M., Yang, F., Leu, N. A., Landaiche, J., Handel, M. A., Benavente, R., La Salle, S., Wang, P. J. MEIOB exhibits single-stranded DNA-binding and exonuclease activities and is essential for meiotic recombination. Nature Commun. 4: 2788, 2013. Note: Electronic Article.

Uniprot ID: Q8N635

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 49 kDa

Calculated Molecular Weight: 49 kDa

Gene ID: 254528

Protein Name: Meiosis-specific with OB domain-containing protein

Gene Full Name: meiosis specific with OB-fold

Synonyms: MEIOB; C16orf73; Meiosis-specific with OB domain-containing protein; EC 3.1.-.-